THIS ITEM HAS BEEN REPLACED WITH Bionova Ultra Rapid Self-Contained Biological Indicator for Steam
Tuttnauer Ultra-Rapid, Biological Indicators for Steam Sterilizers, 20 Minute Results, 50/box
Important: 20-min Tuttnauer BI’s can
ONLY be used for 132C-135C (or 270F-275F)
Be compliant. Avoid long turn-around times on spore tests. Tuttnauer's new biological indicators prove sterilizer performance in just 20 minutes! Recommended for use with Tuttnauer MiniBio Auto-Reader.
Compatible with Class S Gravity and Class B Pre-Post Vacuum autoclaves.
Tuttnauer WTL198-0072
Features:
Self-contained biological indicator
inoculated with viable 106 Geobacillus
stearothermophilus bacterial spores
Spore Tests Results in 20 minutes!
On each BI is a chemical process
indicator that changes color from pink to
brown when exposed to steam
Easy to use and 100% accurate
50 biological indicators per pack
How to Use the BI-TUR biological indicator for steam sterilization:1. Identify
the WTL198-0072 SCBI by writing the sterilizer number (in case of having more
than one), load number and processing date on the label.
2. Pack the
SCBI along with materials to be sterilized in an appropriate package according
to recommended sterilization practices. Place the package in those areas which
are considered most inaccessible for the sterilizing agent (e.g., the center of
the load and areas near the door).
3. Sterilize
as usual.
4. After the
sterilization process has finished, open the sterilizer door, wait five minutes
and remove the SCBI from the package.
CAUTION: Wear safety glasses and gloves when removing the WTL198-0072 SCBI from
the sterilized package. WARNING: Do not crush or handle the SCBI excessively,
since this might cause the glass ampoule to burst. Let the SCBI cool down until
it reaches room temperature.
5. Check the
Process Indicator on SCBI label. A color change to brown indicates that the
SCBI has been exposed to Steam.
IMPORTANT: this color change does not
evidence the process effectiveness to achieve sterility. If the Process
Indicator color has not changed, check the sterilization process.
6. Press the
lid to seal the tube. Crush the ampoule contained in the SCBI with an
individual ampoule crusher or with the ampoule crusher placed within the
incubator´s incubation area. Then shake the tube down vigorously, with
movements similar to those performed to lower the temperature in a mercury
thermometer, until the medium reaches the base of the tube and soaks the spore
carrier entirely. Finally, place the SCBI in the incubator. IMPORTANT: Use a
non-sterilized SCBI as a positive control in order to ensure that correct
incubation conditions were met; capability of medium to promote rapid growth;
viability of spores has not been altered due to improper storage temperature,
humidity or proximity to chemicals and proper functioning of Auto-Reader
Incubators. Both, the positive control indicator and the processed indicator,
should belong to the same batch.
7. Incubate the processed indicator and the
positive control indicator in Auto-Reader Incubators for a maximum of 20
minutes at (60 ± 2) °C for ultra rapid readout.
Readout Times:
Ultra Rapid
readout: 20 minutes
The Ultra Rapid Readout must be carried out in the
Auto-Reader Incubators. Fluorescence is emitted after the reader
stimulates the spore carrier with UV light. Final readout of negative results is readily
available after 20 minutes of incubation. Fluorescence readout is an indirect
measure of the germination and growth of Geobacillus stearothermophilus spores,
which have survived the sterilization process (positive result). Furthermore, a
failure in the sterilization process can also become evident by culture medium
color change. Due to the high sensitivity of the fluorescence results at 20
minutes, conventional incubation for color change of WTL198-0072 is not an
advantage.
Visual
Confirmation: 48 hours
Optionally, you can perform a visual color-change
confirmation after a 48-hour incubation. If the sterilization process has not
been successful, culture medium will turn yellow during incubation at 60 ºC,
indicating the presence of living spores. If sterilization was successful,
culture medium will remain purple after incubation. The positive control must
show a color change from purple to yellow for results to be valid.
Readout time
7 days
A 7-day readout is optional and may be routinely performed. This is an initial
validation of the 20-minute readout. Fluorescence results are compared to the
7-day visual readouts.
NOTE: If 7-day readout is performed, a humidified environment will be required
to prevent medium from drying out.
Read the BI-TUR Instructional Manual before use.
Learn More about the MiniBio Auto Read in our Learning Lab
