Setting Up Your Auto-reader:
1. Place MiniBio Auto-reader on a stable surface, away from extreme conditions.
2. Ensure a 10 cm space from the closest wall.
3. Connect to a secure electrical outlet.
4. Avoid liquid exposure; if spilled, disconnect and dry immediately.
5. Confirm all reading positions are empty before turning on.
Using Your Auto-reader:
1. Power on by connecting the AC plug and jack.
2. Press temperature button for 3 seconds, select desired temperature.
3. Wait for temperature stability indicator to stop blinking.
4. Choose incubation time, place the SCBI, and incubation begins automatically.
5. Wait for completion; green light for negative, red for positive. Press alarm to cancel (if needed).
6. Do not remove SCBI until the result ticket is printed.
Disposal:
Dispose of SCBIs following your country's waste-disposal guidelines.
Connectivity:
Connect MiniBio Auto-reader to a PC via USB for Bionova Traceability Software. Refer to the User’s Manual for details.
Compatibility and Programs:
Explore available incubation programs in the Bionova MiniBio Auto-reader User’s Manual or Compatibility Matrix.
Ultra Rapid Self-Contained Biological Indicator- 50 Biological Indicators per box
Storage Guidelines
- Store in a dark place within the temperature range of 10-30 °C and 30-80% relative humidity.
- Avoid freezing and keep biological indicators away from sterilizing agents or other chemical products.
Instructions for Use: Bionova Ultra Rapid Biological Indicators
Labeling SCBI:
- Identify SCBI with sterilizer number, load number, and processing date.
Packaging and Placement:
- Pack SCBI with materials for sterilization, placing it in less exposed areas.
Sterilization Process:
- Carry out standard sterilization procedures.
Post-Sterilization Steps:
- After sterilization, open the door, wait five minutes, and carefully remove SCBI.
- Caution: Wear safety glasses and gloves.
- Warning: Avoid excessive handling to prevent glass ampoule bursting. Let SCBI cool to room temperature.
Process Indicator Check:
- Inspect the Process Indicator on SCBI label. Brown color indicates exposure to steam.
- Note: Color change does not confirm sterility; further checks are needed if no change.
Ampoule Crushing and Incubation:
- Seal the tube's lid and crush the ampoule using an ampoule crusher.
- Shake the tube to soak the spore carrier completely.
- Place SCBI in the incubator.
- Important: Use a non-sterilized SCBI as a positive control daily. Ensure both controls are from the same batch.
Incubation and Readout:
- Incubate processed and positive control indicators for a maximum of 20 minutes at (60 ± 2) °C.
- Note: Incubation should not exceed a 7-day period after sterilization.
- Failure in sterilization is indicated by fluorescence detection.
- Negative result if no fluorescence at 20 minutes.
- Positive control must show positive fluorescence readout.
- Record positive results and discard SCBI promptly as instructed.
- WARNING: Do not use SCBI to control sterilization processes other than those specified.
- WARNING: Place one or more SCBI in hard-to-reach areas to ensure complete chamber sterilization. Evaluate load configurations to identify and place SCBI in all such locations.
- WARNING: Do not reuse SCBI.
- WARNING: Do not reuse the sterilizer until the SCBI test result is negative.
