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Self-Contained Spore Ampoules to Monitor Liquid Steam Sterilization, Log 4 spore population, Size: 47mm X 10.6mm x 1ml (volume), 50/box

Price: $220.00

Availability: OVERNIGHT SHIPPING ONLY
Product Code: BI-SA-L4

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Description

Technical Specs

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Self-contained Mini Spore Ampules for Monitoring Liquid Steam Processes, with Log 5 spore population.

Important: This item must be shipped UPS OVERNIGHT on ice packs

Size: 40mm x 6.5mm diam. x 0.7ml (volume)
50 per box

Biological indicators can be used in Steam Autoclaves at 121°C to 137°C for monitoring liquid steam processes and dry loads. Must be used with Bionova IC10/20 incubator. For laboratory or industrial use only.

Image	Product Code	Description	Spore Population	Size	Volume	Quantity	Price/box
	BI-MSA-ATM05	Mini Spore Ampoule	Log 5	40mm x 6.5mm diam	0.7ml	50/box plus	$220
	BI-MSA-ATM06	Mini Spore Ampoule	Log 6	40mm x 6.5mm diam	0.7ml	50/box plus	$220
	BI-SA-L4	Spore Ampoule	Log 4	47mm x 10.6mm	1ml	50/box plus1	$220
	BI-SA-L5	Spore Ampoule	Log 5	47mm x 10.6mm	1ml	50/box plus	$220
	BI-SA-L6	Spore Ampoule	Log 6	47mm x 10.6mm	1ml	50/box plus	$339

Call for quantity discounts 877-561-0500

Note: Spore ampoules must be kept refrigerated and ship overnight. These are specially made for monitoring liquid sterilization in steam autoclaves.

Must be used with Bionova IC10/20 incubator sold at Duraline BioSystems, Inc

Instructions for Use:
Place Mini Spore Ampules inside representative materials (containers of liquid) or within the autoclave chamber directly. Package or wrap product as usual, if applicable. Locate product or Mini Spore Ampules in most difficult location to sterilize, as outlined in your specific sterilization validation protocol or according to standard operating procedure. Run the cycle. After sterilization or exposure, remove Mini Spore Ampules or product from sterilizer. Allow product or Spore Ampules to cool to the touch. No activation is required.

Incubation: Place the processed Mini Spore Ampules, one Negative Control Ampule and one Positive Control (unprocessed Mini Spore Ampule) in a vertical position in an incubator at 60°C to 65°C for a minimum of 48 hours.

Monitoring: Examine the Mini Spore Ampules daily during incubation. Record observations. All positive Mini Spore Ampules should be disposed of immediately. Do not continue to incubate a positive Ampule. Continued growth may result in metabolism of amino acids in the absence of sugars, causing the pH to rise and result in color reversion that is visibly darker than a sterile unit. These should be considered as positive for growth (turbidity will be present).

Interpretation: Negative Control: The Negative Control Ampule should not exhibit a color change to Yellow and/or demonstrate turbidity. Utilize the Negative Control as a color comparison for the exposed Mini Spore Ampules, where applicable.

Positive Control Mini Spore Ampule: The Positive Control Ampule should exhibit a color change to Yellow and/or demonstrate turbidity. Utilize the Positive Control as a color comparison for the exposed Ampules, where applicable. If the positive control does not demonstrate a Yellow color and/or turbidity, the results for the test Ampules should not be considered valid. Verify incubation conditions were met.

Test Mini Spore Ampules: A passing sterilization cycle is indicated by a test Spore Ampule which retains its original Purple color and is free from turbidity. A failed sterilization cycle is indicated by turbidity and/or a color change to Yellow.

For unexpected positives, it is recommended that a Gram stain be performed. Gram positive rods are indicative for the indicator organism.

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